Human IL-20/Interleukin-20 ELISA Kit PicoKine®

Interleukin-20 (IL-20) is a protein belonging to the IL-10 family of cytokines. IL-20 is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. By radiation hybrid analysis, the IL20 gene was mapped to chromosome 1q32, where it is tightly linked to the IL10, IL19, and MDA7 genes within a 195-kb region, the IL10 family cytokine cluster. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 97 97 98 99 97 0.82 0.8% Sample 2 693 639 709 709 687 28.75 4.1% Sample 3 1747 1963 1758 1970 1859 107.09 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 97 97 98 99 97 0.82 0.8% Sample 1 97 97 98 99 97 0.82 0.8% Sample 2 693 639 709 709 687 28.75 4.1% Sample 2 693 639 709 709 687 28.75 4.1% Sample 3 1747 1963 1758 1970 1859 107.09 5.7% Sample 3 1747 1963 1758 1970 1859 107.09 5.7%

Expressed in most tissues and five major cell types: epithelial cells (primarily skin, buccal mucosa, tongue, nasal mucosa, lung, ureter, breast, prostate, fallopian tube, and adrenal gland), myoepithelial cells (mainly prostate), endothelial cells (mainly in small vessels or capillaries), macrophages, and skeletal muscle. Isoform 2 was detected in the lung tissue only. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 97 693 1747 90 624 1759 Standard deviation 5.72 46.12 132.77 6.12 44.92 138.96 CV (%) 7.6% 6.9% 7.6% 6.8% 7.2% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 97 693 1747 90 624 1759 Standard deviation 5.72 46.12 132.77 6.12 44.92 138.96 CV (%) 7.6% 6.9% 7.6% 6.8% 7.2% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 97 693 1747 90 624 1759 Mean (pg/ml) 97 693 1747 90 624 1759 Standard deviation 5.72 46.12 132.77 6.12 44.92 138.96 Standard deviation 5.72 46.12 132.77 6.12 44.92 138.96 CV (%) 7.6% 6.9% 7.6% 6.8% 7.2% 7.9% CV (%) 7.6% 6.9% 7.6% 6.8% 7.2% 7.9%

Proinflammatory and angiogenic cytokine that may be involved in epidermal function and psoriasis. Angiogenic and proliferative activities are antagonized by IL10. May act through STAT3. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.041 0.088 0.164 0.298 0.579 1.037 1.859 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.041 0.088 0.164 0.298 0.579 1.037 1.859 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.041 0.088 0.164 0.298 0.579 1.037 1.859 O.D. 0.008 0.041 0.088 0.164 0.298 0.579 1.037 1.859

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.