Human CRISP3 ELISA Kit PicoKine®

Human CRISP3 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CRISP3 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 113 130 111 116 8.15 7.0% Sample 2 383 419 441 377 405 26.26 6.4% Sample 3 1477 1600 1623 1582 1570 55.90 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 113 130 111 116 8.15 7.0% Sample 1 110 113 130 111 116 8.15 7.0% Sample 2 383 419 441 377 405 26.26 6.4% Sample 2 383 419 441 377 405 26.26 6.4% Sample 3 1477 1600 1623 1582 1570 55.90 3.5% Sample 3 1477 1600 1623 1582 1570 55.90 3.5%

Synonyms

Cysteine-rich secretory protein 3; CRISP-3; Specific granule protein of 28 kDa; SGP28; CRISP3

Gene Name

CRISP3

Gene ID

10321

UniProt

P54108

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: N21-Y245

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Salivary gland, pancreas and prostate > epididymis, ovary, thymus and colon.

Applications

ELISA

Field of Research

Cancer, Tumor Biomarkers

Standard

100 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine.

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 383 1477 119 408 1764 Standard deviation 4.51 23.75 66.47 5.59 26.11 95.26 CV (%) 4.1% 6.2% 4.5% 4.7% 6.4% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 383 1477 119 408 1764 Standard deviation 4.51 23.75 66.47 5.59 26.11 95.26 CV (%) 4.1% 6.2% 4.5% 4.7% 6.4% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 110 383 1477 119 408 1764 Mean (pg/ml) 110 383 1477 119 408 1764 Standard deviation 4.51 23.75 66.47 5.59 26.11 95.26 Standard deviation 4.51 23.75 66.47 5.59 26.11 95.26 CV (%) 4.1% 6.2% 4.5% 4.7% 6.4% 5.4% CV (%) 4.1% 6.2% 4.5% 4.7% 6.4% 5.4%

Sensitivity

<50 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

27630 MW

Specificity

Natural and recombinant human CRISP3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cysteine-rich secretory protein 3

Subcellular Location

Secreted. In neutrophils, localized in specific granules.

Sequence Similarities

Belongs to the CRISP family.

Protein Name

Cysteine-rich secretory protein 3

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.001 0.085 0.167 0.356 0.711 1.083 1.773 2.149 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.001 0.085 0.167 0.356 0.711 1.083 1.773 2.149 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.001 0.085 0.167 0.356 0.711 1.083 1.773 2.149 O.D. 0.001 0.085 0.167 0.356 0.711 1.083 1.773 2.149

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.