Human CCL23/MPIF-1 ELISA Kit PicoKine®

Human CCL23/MPIF-1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CCL23 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 23 (CCL23), also known as MIP-3 and MPIF-1, is a small cytokine belonging to the CC chemokine family. It is mapped to 17q12. CCL23 is predominantly expressed in lung and liver tissue, but is also found in placenta, bone marrow and some cell lines of myeloid origin. MPIF1 has chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils. It has also been attributed to an inhibitory activity on hematopoietic progenitor cells that give rise to granulocyte and monocyte lineages. Moreover, CCL23 is a ligand for the chemokine receptor CCR1.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 83 91 90 90 88 3.2 3.6% Sample 2 239 230 235 226 232 4.92 2.1% Sample 3 875 855 806 770 826 41.16 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 83 91 90 90 88 3.2 3.6% Sample 1 83 91 90 90 88 3.2 3.6% Sample 2 239 230 235 226 232 4.92 2.1% Sample 2 239 230 235 226 232 4.92 2.1% Sample 3 875 855 806 770 826 41.16 4.9% Sample 3 875 855 806 770 826 41.16 4.9%

Synonyms

C-C motif chemokine 23; CK-beta-8; CKB-8; Macrophage inflammatory protein 3; MIP-3; Myeloid progenitor inhibitory factor 1; MPIF-1; Small-inducible cytokine A23; CCL23 (19-99) ; CCL23 (22-99) ; CCL23 (27-99) ; CCL23 (30-99) ; CCL23; MIP3, MPIF1, SCYA23

Gene Name

CCL23

Gene ID

6368

UniProt

P55773

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: R22-N120

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23 (19-99), CCL23 (22-99), CCL23 (27-99), CCL23 (30-99) are found in high levels in synovial fluids from rheumatoid patients. .

Applications

ELISA

Field of Research

Chemokines, G Protein Signaling, Immunology, Innate Immunity, Macrophage/Inflammation, Signal Transduction, Signaling Pathway

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 83 239 875 85 246 848 Standard deviation 9%%2 18.16 66.5%5 5.52 22.14 78.86 CV (%) 9%% 7.6% 7.8% 6.5% 9% 9.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 83 239 875 85 246 848 Standard deviation 9%%2 18.16 66.5%5 5.52 22.14 78.86 CV (%) 9%% 7.6% 7.8% 6.5% 9% 9.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 83 239 875 85 246 848 Mean (pg/ml) 83 239 875 85 246 848 Standard deviation 9%%2 18.16 66.5%5 5.52 22.14 78.86 Standard deviation 9%%2 18.16 66.5%5 5.52 22.14 78.86 CV (%) 9%% 7.6% 7.8% 6.5% 9% 9.3% CV (%) 9%% 7.6% 7.8% 6.5% 9% 9.3%

Sensitivity

<2 pg/mL

Function

Shows chemotactic activity for monocytes, resting T- lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23 (19-99), CCL23 (22-99), CCL23 (27-99), CCL23 (30-99) are more potent chemoattractants than the small-inducible cytokine A23. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

13411 MW

Specificity

Natural and recombinant human CCL23

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-C motif chemokine 23

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the intercrine beta (chemokine CC) family.

Protein Name

C-C motif chemokine 23

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.041 0.133 0.2 0.336 0.57 1.023 1.828 2.901 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.041 0.133 0.2 0.336 0.57 1.023 1.828 2.901 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.041 0.133 0.2 0.336 0.57 1.023 1.828 2.901 O.D. 0.041 0.133 0.2 0.336 0.57 1.023 1.828 2.901

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.