Human complement C5a ELISA Kit PicoKine®

C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 89 97 98 84 92 5.78 6.2% Sample 2 285 261 256 258 265 11.68 4.4% Sample 3 1037 990 926 1010 990 40.93 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 89 97 98 84 92 5.78 6.2% Sample 1 89 97 98 84 92 5.78 6.2% Sample 2 285 261 256 258 265 11.68 4.4% Sample 2 285 261 256 258 265 11.68 4.4% Sample 3 1037 990 926 1010 990 40.93 4.1% Sample 3 1037 990 926 1010 990 40.93 4.1%

Complement C5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; Complement C5 beta chain; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5 alpha' chain; C5; CPAMD4

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 89 285 1037 84 285 1015 Standard deviation 4.09 17.1 67.46 6.82 21.09 69.02 CV (%) 4.6% 6% 6.4% 6.1% 7.4% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 89 285 1037 84 285 1015 Standard deviation 4.09 17.1 67.46 6.82 21.09 69.02 CV (%) 4.6% 6% 6.4% 6.1% 7.4% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 89 285 1037 84 285 1015 Mean (pg/ml) 89 285 1037 84 285 1015 Standard deviation 4.09 17.1 67.46 6.82 21.09 69.02 Standard deviation 4.09 17.1 67.46 6.82 21.09 69.02 CV (%) 4.6% 6% 6.4% 6.1% 7.4% 6.8% CV (%) 4.6% 6% 6.4% 6.1% 7.4% 6.8%

Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.043 0.102 0.145 0.241 0.405 0.713 1.219 2.039 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.043 0.102 0.145 0.241 0.405 0.713 1.219 2.039 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.043 0.102 0.145 0.241 0.405 0.713 1.219 2.039 O.D. 0.043 0.102 0.145 0.241 0.405 0.713 1.219 2.039

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.