Human ANGPTL4 / Angiopoietin Like 4 / ARP4 ELISA Kit PicoKine®

Angiopoietin-related protein 4 (Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1114 1122 1295 1157 1172 72.83 6.2% Sample 2 4583 4850 5176 4540 4787 253.92 5.3% Sample 3 20920 21440 20536 18895 20447 952.15 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1114 1122 1295 1157 1172 72.83 6.2% Sample 1 1114 1122 1295 1157 1172 72.83 6.2% Sample 2 4583 4850 5176 4540 4787 253.92 5.3% Sample 2 4583 4850 5176 4540 4787 253.92 5.3% Sample 3 20920 21440 20536 18895 20447 952.15 4.6% Sample 3 20920 21440 20536 18895 20447 952.15 4.6%

Angiopoietin-related protein 4; Angiopoietin-like protein 4; Hepatic fibrinogen/angiopoietin-related protein; HFARP; ANGPTL4; ARP4, HFARP, PGAR; PP1158, PSEC0166, UNQ171/PRO197

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Cholesterol Metabolism, Growth Factors, Hypoxia, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Metabolism Processes, Pathways and Processes, Response To Hypoxia

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1114 4583 20920 1120 4790 21489 Standard deviation 73.52 256.64 941.4 79.52 320.93 988.49 CV (%) 6.6% 5.6% 4.5% 7.1% 6.7% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1114 4583 20920 1120 4790 21489 Standard deviation 73.52 256.64 941.4 79.52 320.93 988.49 CV (%) 6.6% 5.6% 4.5% 7.1% 6.7% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1114 4583 20920 1120 4790 21489 Mean (pg/ml) 1114 4583 20920 1120 4790 21489 Standard deviation 73.52 256.64 941.4 79.52 320.93 988.49 Standard deviation 73.52 256.64 941.4 79.52 320.93 988.49 CV (%) 6.6% 5.6% 4.5% 7.1% 6.7% 4.6% CV (%) 6.6% 5.6% 4.5% 7.1% 6.7% 4.6%

Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is ly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM) . The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Secreted . Secreted, extracellular space, extracellular matrix . The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4 (By similarity) . .

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.018 0.054 0.105 0.189 0.458 0.759 1.468 2.067 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.018 0.054 0.105 0.189 0.458 0.759 1.468 2.067 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.018 0.054 0.105 0.189 0.458 0.759 1.468 2.067 O.D. 0.018 0.054 0.105 0.189 0.458 0.759 1.468 2.067

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.