Rat DLL4 ELISA Kit PicoKine®

Delta like ligand 4 is a protein that in humans is encoded by the DLL4 gene. It is mapped to 15q15.1. This gene is a homolog of the Drosophila delta gene. DLL4 is a transmembrane ligand for Notch receptors that shows restricted expression to endothelial cells (ECs), in particular to arteries and capillaries, and is involved in vascular development, and it also appeared to be a major trigger of Notch receptor activities previously implicated in arterial and vascular development. DLL4 acts as a negative regulator of tumor angiogenesis, its blockade results in the striking uncoupling of tumor growth from vessel density, presenting a novel therapeutic approach even for tumors resistant to anti-VEGF therapies. In addition to it, this gene also plays an important role in promoting Th17 effector activity during mycobacterial challenge.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 34 35 35 34 0.5 1.4% Sample 2 212 221 209 203 211 6.49 3% Sample 3 425 416 448 431 430 11.68 2.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 34 35 35 34 0.5 1.4% Sample 1 34 34 35 35 34 0.5 1.4% Sample 2 212 221 209 203 211 6.49 3% Sample 2 212 221 209 203 211 6.49 3% Sample 3 425 416 448 431 430 11.68 2.7% Sample 3 425 416 448 431 430 11.68 2.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 212 425 37 205 382 Standard deviation 2.27 9.96 19.12 2.51 11.89 17.95 CV (%) 6.7% 4.7% 4.5% 6.8% 5.8% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 212 425 37 205 382 Standard deviation 2.27 9.96 19.12 2.51 11.89 17.95 CV (%) 6.7% 4.7% 4.5% 6.8% 5.8% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 212 425 37 205 382 Mean (pg/ml) 34 212 425 37 205 382 Standard deviation 2.27 9.96 19.12 2.51 11.89 17.95 Standard deviation 2.27 9.96 19.12 2.51 11.89 17.95 CV (%) 6.7% 4.7% 4.5% 6.8% 5.8% 4.7% CV (%) 6.7% 4.7% 4.5% 6.8% 5.8% 4.7%

Involved in the Notch signaling pathway as Notch ligand. Activates NOTCH1 and NOTCH4 (PubMed:25700513) . Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types. During spinal cord neurogenesis, inhibits V2a interneuron fate (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.014 0.093 0.154 0.268 0.473 0.822 1.388 1.924 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.014 0.093 0.154 0.268 0.473 0.822 1.388 1.924 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.014 0.093 0.154 0.268 0.473 0.822 1.388 1.924 O.D. 0.014 0.093 0.154 0.268 0.473 0.822 1.388 1.924

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.