Human CD23/FCER2 ELISA Kit PicoKine®

CD23, also known as Fc epsilon RII, or FcεRII, is the low-affinity receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the FCE2 gene was assigned to 19p13.3. CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 71 62 62 64 4.06 6.3% Sample 2 194 218 229 235 219 15.66 7.1% Sample 3 981 870 950 851 913 54.05 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 71 62 62 64 4.06 6.3% Sample 1 61 71 62 62 64 4.06 6.3% Sample 2 194 218 229 235 219 15.66 7.1% Sample 2 194 218 229 235 219 15.66 7.1% Sample 3 981 870 950 851 913 54.05 5.9% Sample 3 981 870 950 851 913 54.05 5.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 194 981 55 207 899 Standard deviation 2.8 7.95 64.74 3.41 9.93 71.02 CV (%) 4.6% 4.1% 6.6% 6.2% 4.8% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 194 981 55 207 899 Standard deviation 2.8 7.95 64.74 3.41 9.93 71.02 CV (%) 4.6% 4.1% 6.6% 6.2% 4.8% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 61 194 981 55 207 899 Mean (pg/ml) 61 194 981 55 207 899 Standard deviation 2.8 7.95 64.74 3.41 9.93 71.02 Standard deviation 2.8 7.95 64.74 3.41 9.93 71.02 CV (%) 4.6% 4.1% 6.6% 6.2% 4.8% 7.9% CV (%) 4.6% 4.1% 6.6% 6.2% 4.8% 7.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.056 0.108 0.199 0.356 0.608 1.155 2.001 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.056 0.108 0.199 0.356 0.608 1.155 2.001 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.056 0.108 0.199 0.356 0.608 1.155 2.001 O.D. 0.002 0.056 0.108 0.199 0.356 0.608 1.155 2.001

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.