Human KIT / SCFR / CD117 / c Kit ELISA Kit PicoKine®

SCFR (Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 339 296 321 331 321 16.17 5% Sample 2 1218 1298 1258 1193 1241 39.9 3.2% Sample 3 4619 4300 4189 4585 4423 183.4 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 339 296 321 331 321 16.17 5% Sample 1 339 296 321 331 321 16.17 5% Sample 2 1218 1298 1258 1193 1241 39.9 3.2% Sample 2 1218 1298 1258 1193 1241 39.9 3.2% Sample 3 4619 4300 4189 4585 4423 183.4 4.1% Sample 3 4619 4300 4189 4585 4423 183.4 4.1%

Mast/stem cell growth factor receptor Kit; SCFR;2.7.10.1; Piebald trait protein; PBT; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD117; KIT; SCFR

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level) . Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells. .

Cancer, Cell Type Markers, Cytokines, Developmental Biology, Germ Cell Markers, Germline Stem Cells, Hematopoietic Progenitors, Immunology, Myeloid, Neuroscience, Neurotransmission, Oncoproteins, Oncoproteins/Suppressors, Organogenesis, Protein Phosphorylation, Receptor Tyrosine Kinases, Receptors / Channels, Reproduction, Signal Transduction, Stem Cells, Surface Molecules, Tags & Cell Markers, Tumor Associated, Tyrosine Kinases

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 339 1218 4619 346 1138 4342 Standard deviation 23.39 64.55 314.09 26.98 60.31 329.99 CV (%) 6.9% 5.3% 6.8% 7.8% 5.3% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 339 1218 4619 346 1138 4342 Standard deviation 23.39 64.55 314.09 26.98 60.31 329.99 CV (%) 6.9% 5.3% 6.8% 7.8% 5.3% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 339 1218 4619 346 1138 4342 Mean (pg/ml) 339 1218 4619 346 1138 4342 Standard deviation 23.39 64.55 314.09 26.98 60.31 329.99 Standard deviation 23.39 64.55 314.09 26.98 60.31 329.99 CV (%) 6.9% 5.3% 6.8% 7.8% 5.3% 7.6% CV (%) 6.9% 5.3% 6.8% 7.8% 5.3% 7.6%

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.07 0.117 0.2 0.384 0.777 1.422 2.095 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.07 0.117 0.2 0.384 0.777 1.422 2.095 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.07 0.117 0.2 0.384 0.777 1.422 2.095 O.D. 0.024 0.07 0.117 0.2 0.384 0.777 1.422 2.095

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.