Mouse IL-21/Interleukin-21 ELISA Kit PicoKine®

Interleukin-21 is a protein that in humans is encoded by the IL21 gene. Interleukin 21 is a cytokine that has potent regulatory effects on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. By radiation hybrid analysis, the IL21 gene is mapped to chromosome 4q26-q27, near the IL2 gene. IL21 stimulates B-cell proliferation in an anti-CD40-dependent manner but inhibits proliferation stimulated by IL4 plus anti-IgM. The biologic effects of IL21 on NK cells differ between species, with human IL21 enhancing NK cell proliferation and murine IL21 inhibiting NK cell proliferation. IL21 is a cytokine highly expressed by mouse T (H) 17 cells.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 176 199 176 180 182 9.52 5.2% Sample 2 862 737 773 850 805 52.25 6.4% Sample 3 1322 1296 1226 1149 1248 67.2 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 176 199 176 180 182 9.52 5.2% Sample 1 176 199 176 180 182 9.52 5.2% Sample 2 862 737 773 850 805 52.25 6.4% Sample 2 862 737 773 850 805 52.25 6.4% Sample 3 1322 1296 1226 1149 1248 67.2 5.3% Sample 3 1322 1296 1226 1149 1248 67.2 5.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 176 862 1322 188 946 1262 Standard deviation 9.32 43.96 96.50 9.96 52.97 107.27 CV (%) 5.3% 8.5% 7.3% 5.3% 5.6% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 176 862 1322 188 946 1262 Standard deviation 9.32 43.96 96.50 9.96 52.97 107.27 CV (%) 5.3% 8.5% 7.3% 5.3% 5.6% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 176 862 1322 188 946 1262 Mean (pg/ml) 176 862 1322 188 946 1262 Standard deviation 9.32 43.96 96.50 9.96 52.97 107.27 Standard deviation 9.32 43.96 96.50 9.96 52.97 107.27 CV (%) 5.3% 8.5% 7.3% 5.3% 5.6% 8.5% CV (%) 5.3% 8.5% 7.3% 5.3% 5.6% 8.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.042 0.08 0.14 0.246 0.46 0.894 1.869 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.042 0.08 0.14 0.246 0.46 0.894 1.869 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.042 0.08 0.14 0.246 0.46 0.894 1.869 O.D. 0.008 0.042 0.08 0.14 0.246 0.46 0.894 1.869

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.