Mouse CRP / C Reactive Protein / PTX1 PicoKine® Quick ELISA Kit

C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 254 243 280 249 256 13.82 5.4% Sample 2 1752 1563 1416 1662 1598 97.47 6.1% Sample 3 6770 5545 5924 6118 6089 395.78 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 254 243 280 249 256 13.82 5.4% Sample 1 254 243 280 249 256 13.82 5.4% Sample 2 1752 1563 1416 1662 1598 97.47 6.1% Sample 2 1752 1563 1416 1662 1598 97.47 6.1% Sample 3 6770 5545 5924 6118 6089 395.78 6.5% Sample 3 6770 5545 5924 6118 6089 395.78 6.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 254 1752 6770 265 1851 6930 Standard deviation 11.93 94.6 453.59 12.98 105.5 512.82 CV (%) 4.7% 5.4% 6.7% 4.9% 5.7% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 254 1752 6770 265 1851 6930 Standard deviation 11.93 94.6 453.59 12.98 105.5 512.82 CV (%) 4.7% 5.4% 6.7% 4.9% 5.7% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 254 1752 6770 265 1851 6930 Mean (pg/ml) 254 1752 6770 265 1851 6930 Standard deviation 11.93 94.6 453.59 12.98 105.5 512.82 Standard deviation 11.93 94.6 453.59 12.98 105.5 512.82 CV (%) 4.7% 5.4% 6.7% 4.9% 5.7% 7.4% CV (%) 4.7% 5.4% 6.7% 4.9% 5.7% 7.4%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.071 0.139 0.260 0.384 0.666 1.120 1.877 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.071 0.139 0.260 0.384 0.666 1.120 1.877 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.071 0.139 0.260 0.384 0.666 1.120 1.877 O.D. 0.035 0.071 0.139 0.260 0.384 0.666 1.120 1.877

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.