Mouse CRP / C Reactive Protein / PTX1 PicoKine® Quick ELISA Kit

Mouse CRP / C Reactive Protein / PTX1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Crp in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 254 243 280 249 256 13.82 5.4% Sample 2 1752 1563 1416 1662 1598 97.47 6.1% Sample 3 6770 5545 5924 6118 6089 395.78 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 254 243 280 249 256 13.82 5.4% Sample 1 254 243 280 249 256 13.82 5.4% Sample 2 1752 1563 1416 1662 1598 97.47 6.1% Sample 2 1752 1563 1416 1662 1598 97.47 6.1% Sample 3 6770 5545 5924 6118 6089 395.78 6.5% Sample 3 6770 5545 5924 6118 6089 395.78 6.5%

Synonyms

C-Reactive Protein/CRP; C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895

Gene Name

CST3

Gene ID

12944

UniProt

P14847

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: H20-S225

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Atherosclerosis, Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 254 1752 6770 265 1851 6930 Standard deviation 11.93 94.6 453.59 12.98 105.5 512.82 CV (%) 4.7% 5.4% 6.7% 4.9% 5.7% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 254 1752 6770 265 1851 6930 Standard deviation 11.93 94.6 453.59 12.98 105.5 512.82 CV (%) 4.7% 5.4% 6.7% 4.9% 5.7% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 254 1752 6770 265 1851 6930 Mean (pg/ml) 254 1752 6770 265 1851 6930 Standard deviation 11.93 94.6 453.59 12.98 105.5 512.82 Standard deviation 11.93 94.6 453.59 12.98 105.5 512.82 CV (%) 4.7% 5.4% 6.7% 4.9% 5.7% 7.4% CV (%) 4.7% 5.4% 6.7% 4.9% 5.7% 7.4%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Mouse Crp

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-reactive protein, pentraxin-related

Protein Name

C-reactive protein

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.071 0.139 0.260 0.384 0.666 1.120 1.877 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.071 0.139 0.260 0.384 0.666 1.120 1.877 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.071 0.139 0.260 0.384 0.666 1.120 1.877 O.D. 0.035 0.071 0.139 0.260 0.384 0.666 1.120 1.877

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.