Human PTX3/Pentraxin 3 PicoKine® Quick ELISA Kit

Human PTX3/Pentraxin 3 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human PTX3 in cell culture supernatants, serum, plasma (EDTA) and saliva. . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

PTX3 (Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25.32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complementactivation and facilitates pathogen recognition by macrophages and DCs.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 687 618 703 627 658 36.19 5.5% Sample 2 1807 1579 1636 1784 1701 81.64 4.8% Sample 3 9209 8917 8805 8754 8921 330.07 3.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 687 618 703 627 658 36.19 5.5% Sample 1 687 618 703 627 658 36.19 5.5% Sample 2 1807 1579 1636 1784 1701 81.64 4.8% Sample 2 1807 1579 1636 1784 1701 81.64 4.8% Sample 3 9209 8917 8805 8754 8921 330.07 3.7% Sample 3 9209 8917 8805 8754 8921 330.07 3.7%

Synonyms

Pentraxin 3/TSG-14|alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14

Gene Name

CST3

Gene ID

5806

UniProt

P26022

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E18-S381

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Atherosclerosis, Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (EDTA) and saliva.

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 687 1807 9209 814 1923 9534 Standard deviation 37.09 110.22 635.42 56.16 138.45 762.72 CV (%) 5.4% 6.1% 6.9% 6.9% 7.2% 8.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 687 1807 9209 814 1923 9534 Standard deviation 37.09 110.22 635.42 56.16 138.45 762.72 CV (%) 5.4% 6.1% 6.9% 6.9% 7.2% 8.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 687 1807 9209 814 1923 9534 Mean (pg/ml) 687 1807 9209 814 1923 9534 Standard deviation 37.09 110.22 635.42 56.16 138.45 762.72 Standard deviation 37.09 110.22 635.42 56.16 138.45 762.72 CV (%) 5.4% 6.1% 6.9% 6.9% 7.2% 8.0% CV (%) 5.4% 6.1% 6.9% 6.9% 7.2% 8.0%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human PTX3

Anticoagulant

EDTA

Gene Name Synonym

Pentraxin 3

Protein Name

Pentraxin-related protein PTX3

Sample Data

Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.033 0.075 0.154 0.281 0.498 0.937 1.559 2.039 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.033 0.075 0.154 0.281 0.498 0.937 1.559 2.039 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.033 0.075 0.154 0.281 0.498 0.937 1.559 2.039 O.D. 0.033 0.075 0.154 0.281 0.498 0.937 1.559 2.039

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.