Human Rantes PicoKine® Quick ELISA Kit

Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 35 39 38 36 1.78 4.9% Sample 2 166 149 157 169 160 7.85 4.9% Sample 3 442 377 390 418 406 25.17 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 35 39 38 36 1.78 4.9% Sample 1 35 35 39 38 36 1.78 4.9% Sample 2 166 149 157 169 160 7.85 4.9% Sample 2 166 149 157 169 160 7.85 4.9% Sample 3 442 377 390 418 406 25.17 6.1% Sample 3 442 377 390 418 406 25.17 6.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 166 442 31 169 476 Standard deviation 1.71 11.45 22.54 1.58 12.67 33.32 CV (%) 4.9% 6.9% 7%% 7%% 7.5% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 166 442 31 169 476 Standard deviation 1.71 11.45 22.54 1.58 12.67 33.32 CV (%) 4.9% 6.9% 7%% 7%% 7.5% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 166 442 31 169 476 Mean (pg/ml) 35 166 442 31 169 476 Standard deviation 1.71 11.45 22.54 1.58 12.67 33.32 Standard deviation 1.71 11.45 22.54 1.58 12.67 33.32 CV (%) 4.9% 6.9% 7%% 7%% 7.5% 7% CV (%) 4.9% 6.9% 7%% 7%% 7.5% 7%

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.051 0.099 0.207 0.450 0.934 1.769 2.497 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.051 0.099 0.207 0.450 0.934 1.769 2.497 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.051 0.099 0.207 0.450 0.934 1.769 2.497 O.D. 0.007 0.051 0.099 0.207 0.450 0.934 1.769 2.497

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.