Human Rantes PicoKine® Quick ELISA Kit

Human Rantes PicoKine® Quick ELISA Kit (120 minutes, 96 Tests) . Quantitate Human CCL5 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 35 39 38 36 1.78 4.9% Sample 2 166 149 157 169 160 7.85 4.9% Sample 3 442 377 390 418 406 25.17 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 35 39 38 36 1.78 4.9% Sample 1 35 35 39 38 36 1.78 4.9% Sample 2 166 149 157 169 160 7.85 4.9% Sample 2 166 149 157 169 160 7.85 4.9% Sample 3 442 377 390 418 406 25.17 6.1% Sample 3 442 377 390 418 406 25.17 6.1%

Synonyms

C-C motif chemokine ; CCL5 ; hCG_32592

Gene Name

CCL5

Gene ID

6352

UniProt

D0EI67

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S24-S91

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 166 442 31 169 476 Standard deviation 1.71 11.45 22.54 1.58 12.67 33.32 CV (%) 4.9% 6.9% 7%% 7%% 7.5% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 166 442 31 169 476 Standard deviation 1.71 11.45 22.54 1.58 12.67 33.32 CV (%) 4.9% 6.9% 7%% 7%% 7.5% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 166 442 31 169 476 Mean (pg/ml) 35 166 442 31 169 476 Standard deviation 1.71 11.45 22.54 1.58 12.67 33.32 Standard deviation 1.71 11.45 22.54 1.58 12.67 33.32 CV (%) 4.9% 6.9% 7%% 7%% 7.5% 7% CV (%) 4.9% 6.9% 7%% 7%% 7.5% 7%

Sensitivity

<3 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

9990 MW

Specificity

Natural and recombinant human Rantes

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

C-C motif chemokine

Subcellular Location

Secreted .

Protein Name

C-C motif chemokine 5

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.051 0.099 0.207 0.450 0.934 1.769 2.497 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.051 0.099 0.207 0.450 0.934 1.769 2.497 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.051 0.099 0.207 0.450 0.934 1.769 2.497 O.D. 0.007 0.051 0.099 0.207 0.450 0.934 1.769 2.497

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.