Rat GDNF PicoKine® Quick ELISA Kit

Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures. The standard product used in this kit is recombinant rat GDNF, which is a dimmer composed of two chains with 134 amino acids.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 86 80 91 86 4.32 5% Sample 2 280 271 279 263 273 6.86 2.5% Sample 3 983 996 962 999 985 14.57 1.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 86 80 91 86 4.32 5% Sample 1 90 86 80 91 86 4.32 5% Sample 2 280 271 279 263 273 6.86 2.5% Sample 2 280 271 279 263 273 6.86 2.5% Sample 3 983 996 962 999 985 14.57 1.4% Sample 3 983 996 962 999 985 14.57 1.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 280 983 94 264 1071 Standard deviation 4.32 22.12 69.79 5.54 24.55 84.6 CV (%) 4.8% 7.9% 7.1% 5.9% 9.3% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 280 983 94 264 1071 Standard deviation 4.32 22.12 69.79 5.54 24.55 84.6 CV (%) 4.8% 7.9% 7.1% 5.9% 9.3% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 90 280 983 94 264 1071 Mean (pg/ml) 90 280 983 94 264 1071 Standard deviation 4.32 22.12 69.79 5.54 24.55 84.6 Standard deviation 4.32 22.12 69.79 5.54 24.55 84.6 CV (%) 4.8% 7.9% 7.1% 5.9% 9.3% 7.9% CV (%) 4.8% 7.9% 7.1% 5.9% 9.3% 7.9%

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. .

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.048 0.100 0.195 0.408 0.734 1.487 2.112 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.048 0.100 0.195 0.408 0.734 1.487 2.112 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.048 0.100 0.195 0.408 0.734 1.487 2.112 O.D. 0.002 0.048 0.100 0.195 0.408 0.734 1.487 2.112

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.