What is the catalog number for Feline IL-10 ELISA Kit PicoKine®?

The catalog number for Feline IL-10 ELISA Kit PicoKine® is EK2370.

What sizes are available for Feline IL-10 ELISA Kit PicoKine®?

Feline IL-10 ELISA Kit PicoKine® is available in the following size(s): 96 Well With Removable Strips.

Is Feline IL-10 ELISA Kit PicoKine® in stock?

Feline IL-10 ELISA Kit PicoKine® is currently made to order / not held in immediate stock. Contact us for lead time.

Where can I find the safety data sheet (SDS/MSDS) for Feline IL-10 ELISA Kit PicoKine®?

The safety data sheet for Feline IL-10 ELISA Kit PicoKine® is available here: https://www.bosterbio.com/msds?sku=EK2370.

Is this product suitable for live cell imaging?

Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.

Is this product covered by a money-back guarantee?

Yes, we guarantee a full refund or store credit if this product arrives damaged beyond usability, or has a genuine performance issue under proper usage and storage, provided you report it immediately with evidence. Claims after the expiration date or due to improper storage after receipt are excluded.

Can this product be used for diagnostic or therapeutic purposes?

No. This product, like all items we supply, is intended for research laboratory use only and must not be used for diagnostics, therapeutics, treatment, or consumption by humans or animals.

Feline IL-10 ELISA Kit PicoKine®

Feline IL-10 ELISA Kit PicoKine® (96 Tests) . Quantitate Feline IL-10 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that down regulates the immune system. It is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 216 173 173 215 194 10.76 5.5% Sample 2 877 822 939 949 897 26.10 2.9% Sample 3 2140 2834 3514 2729 2804 169.66 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 216 173 173 215 194 10.76 5.5% Sample 1 216 173 173 215 194 10.76 5.5% Sample 2 877 822 939 949 897 26.10 2.9% Sample 2 877 822 939 949 897 26.10 2.9% Sample 3 2140 2834 3514 2729 2804 169.66 6.1% Sample 3 2140 2834 3514 2729 2804 169.66 6.1%

Synonyms

Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; Il10; Il-10

Gene Name

IL10

Gene ID

493683

UniProt

P55029

Reactivity

Feline

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Widely expressed with highest levels in lymphoid tissues. .

Applications

ELISA

Field of Research

Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

125.0-8,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 216 877 2140 150 930 3500 Standard deviation 6.76 18.77 111.07 3.33/td> 50.69 148.40 CV (%) 3.1% 2.1% 5.2% 2.2% 5.5% 4.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 216 877 2140 150 930 3500 Standard deviation 6.76 18.77 111.07 3.33/td> 50.69 148.40 CV (%) 3.1% 2.1% 5.2% 2.2% 5.5% 4.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 216 877 2140 150 930 3500 Mean (pg/ml) 216 877 2140 150 930 3500 Standard deviation 6.76 18.77 111.07 3.33/td> 50.69 148.40 Standard deviation 6.76 18.77 111.07 3.33/td> 50.69 148.40 CV (%) 3.1% 2.1% 5.2% 2.2% 5.5% 4.2% CV (%) 3.1% 2.1% 5.2% 2.2% 5.5% 4.2%

Sensitivity

<15 pg/mL

Function

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

20575 MW

Specificity

Natural and recombinant feline IL-10

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin 10

Subcellular Location

Secreted.

Protein Name

Receptor tyrosine-protein kinase erbB-2

Sample Data

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.052 0.109 0.151 0.235 0.369 0.657 1.208 2.045 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.052 0.109 0.151 0.235 0.369 0.657 1.208 2.045 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.052 0.109 0.151 0.235 0.369 0.657 1.208 2.045 O.D. 0.052 0.109 0.151 0.235 0.369 0.657 1.208 2.045