Human Resistin ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance.1 Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin (for resistance to insulin), that may be the hormone potentially links obesity to diabetes.2 Resistin is overexpressed in human adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.3 The standard product used in this kit is human Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.

Resistin; Adipose tissue-specific secretory factor; ADSF; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; RETN; FIZZ3; HXCP1; RSTN; UNQ407/PRO1199

Expressed in white adipose tissue (at protein level) (PubMed:11201732) . Widely expressed, with particularly strong expression in lung, bone marrow, breast and peripheral blood (PubMed:15248836) . Expressed strongly in bone marrow and at lower levels in lung, but not detected in other tissues (PubMed:15064728) . Isoform 2 is detected in adipose tissue, bone marrow, brain, lung, peripheral blood, placenta and thymus (PubMed:15248836) .

Atherosclerosis, Cardiovascular, Diabetes-associated, Energy Metabolism, Energy Transfer Pathways, Growth Factors/Hormones, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 534 1453 2323 554 1532 2486 Standard deviation 28.84 88.63 109.2 45.98 119.5 200 CV (%) 5.4% 6.1% 4.7% 8.3% 7.8% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 534 1453 2323 554 1532 2486 Standard deviation 28.84 88.63 109.2 45.98 119.5 200 CV (%) 5.4% 6.1% 4.7% 8.3% 7.8% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 534 1453 2323 554 1532 2486 Mean (pg/ml) 534 1453 2323 554 1532 2486 Standard deviation 28.84 88.63 109.2 45.98 119.5 200 Standard deviation 28.84 88.63 109.2 45.98 119.5 200 CV (%) 5.4% 6.1% 4.7% 8.3% 7.8% 8% CV (%) 5.4% 6.1% 4.7% 8.3% 7.8% 8%

Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells (By similarity) . Potentially links obesity to diabetes (By similarity) . Promotes chemotaxis in myeloid cells (PubMed:15064728) .

Catalog number Description Quantity Storage of opened/reconstituted material EZ0581-CA Rabbit anti- human RETN polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0581-DA Biotinylated goat anti- human RETN polyclonal antibody (Detection Antibody) 500 μl, 5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0581-ST Lyophilized recombinant human RETN standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0581-CA Rabbit anti- human RETN polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0581-DA Biotinylated goat anti- human RETN polyclonal antibody (Detection Antibody) 500 μl, 5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0581-ST Lyophilized recombinant human RETN standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0581-CA Rabbit anti- human RETN polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0581-CA Rabbit anti- human RETN polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0581-DA Biotinylated goat anti- human RETN polyclonal antibody (Detection Antibody) 500 μl, 5 μg/mL (recommended dilution 1:100) EZ0581-DA Biotinylated goat anti- human RETN polyclonal antibody (Detection Antibody) 500 μl, 5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0581-ST Lyophilized recombinant human RETN standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK0581-ST Lyophilized recombinant human RETN standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.000 0.121 0.208 0.367 0.660 0.989 1.452 1.990 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.000 0.121 0.208 0.367 0.660 0.989 1.452 1.990 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.000 0.121 0.208 0.367 0.660 0.989 1.452 1.990 O.D. 0.000 0.121 0.208 0.367 0.660 0.989 1.452 1.990

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)