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Anti-Myosin Light Chain 2/MLC-2V/MYL2 Antibody Picoband® Fluoro488 Conjugated

Selected Variant

A03215-3-Fluoro488

Size: 100 µg/Vial

Product Specifications

Background

Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC-2) also known as the regulatory light chain of myosin (RLC) is a protein that in humans is encoded by the MYL2 gene. This gene encodes a major sarcomeric protein in mammalian striated muscle. The encoded protein plays a role in embryonic heart muscle structure and function, while phosphorylation of the encoded protein is involved in cardiac myosin cycling kinetics, torsion and function in adults. Mutations in this gene are associated with hypertrophic cardiomyopathy 10 and infant-onset myopathy.

Synonyms

MYL2; MLC2; Myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; MLC-2v; Cardiac myosin light chain 2; Myosin light chain 2, slow skeletal/ventricular muscle isoform; MLC-2s/v; Ventricular myosin light chain 2

Gene Name

MYL2

Gene ID

4633

UniProt

P10916

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Myosin Light Chain 2/MLC-2V/MYL2, which shares 95.7% amino acid (aa) sequence identity with both mouse and rat Myosin Light Chain 2/MLC-2V/MYL2.

Clonality

Polyclonal

Applications

Flow Cytometry

Purification

Immunogen affinity purified.

Form

Liquid

References & Citations

1. Barth, P. G., Wanders, R. J. A., Ruitenbeek, W., Roe, C., Scholte, H. R., van der Harten, H., van Moorsel, J., Duran, M., Dingemans, K. P. Infantile fibre type disproportion, myofibrillar lysis and cardiomyopathy: a disorder in three unrelated Dutch families. Neuromusc. Disord. 8: 296-304, 1998. 2. Davis, J. S., Hassanzadeh, S., Winitsky, S., Lin, H., Satorius, C., Vemuri, R., Aletras, A. H., Wen, H., Epstein, N. D. The overall pattern of cardiac contraction depends on a spatial gradient of myosin regulatory light chain phosphorylation. Cell 107: 631-641, 2001. 3. Flavigny, J., Richard, P., Isnard, R., Carrier, L., Charron, P., Bonne, G., Forissier, J.-F., Desnos, M., Dubourg, O., Komajda, M., Schwartz, K., Hainque, B. Identification of two novel mutations in the ventricular regulatory myosin light chain gene (MYL2) associated with familial and classical forms of hypertrophic cardiomyopathy. J. Molec. Med. 76: 208-214, 1998.

Storage Conditions

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Calculated Molecular Weight

19 kDa

Applications Notes

6

Gene Name Synonym

Myosin light chain 2

Protein Name

Myosin regulatory light chain 2, ventricular/cardiac muscle isoform

Isotype

Rabbit IgG

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

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