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Anti-Cyclin B1/CCNB1 Antibody Picoband®

Boster Bio Anti-Cyclin B1/CCNB1 Antibody Picoband® catalog # A00745-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

G2/mitotic-specific cyclin-B1 is a protein that in humans is encoded by the CCNB1 gene. It is mapped to 5q13.2. The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle.

Synonyms

G2/mitotic-specific cyclin-B1; CCNB1; CCNB

Gene Name

CCNB1

Gene ID

891

UniProt

P14635

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Cyclin B1/CCNB1 recombinant protein (Position: M1-L383) .

Clonality

Polyclonal

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cancer, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

References & Citations

1. Matsuo, T., Yamaguchi, S., Mitsui, S., Emi, A., Shimoda, F., Okamura, H. Control mechanism of the circadian clock for timing of cell division in vivo. Science 302: 255-259, 2003. 2. Milatovich, A., Francke, U. Human cyclin B1 gene (CCNB1) assigned to chromosome 5 (q13-qter) . Somat. Cell Molec. Genet. 18: 303-307, 1992. 3. Moore, J. D., Kirk, J. A., Hunt, T. Unmasking the S-phase-promoting potential of cyclin B1. Science 300: 987-990, 2003.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

51658 MW

Observed Molecular Weight

55 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Cyclin B1

Subcellular Location

Centrosome. Nucleus. Cytoplasm.

Isotype

Rabbit IgG

Contents

243

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