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Anti-GLB1/Beta-galactosidase Antibody Picoband®

Boster Bio Anti-GLB1/Beta-galactosidase Antibody Picoband® catalog # A01829-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Galactosidase, beta 1, also known as GLB1, is a protein which in humans is encoded by the GLB1 gene. I t is mapped to 3p22.3. This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome.

Synonyms

Beta-galactosidase; Glb1; Glb1_mapped; rCG_25043

Gene Name

Glb1

Gene ID

12091

UniProt

D3ZUM4

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived rat GLB1/Beta-galactosidase recombinant protein (Position: R103-I646) .

Clonality

Polyclonal

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Neuroscience, Stem Cells, Synapse Marker, T Cells, T Lymphocytic Lineage

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.EC:3.2.1.23

References & Citations

1. Shows TB, Scrafford-Wolff L, Brown JA, Meisler M (1978) . Assignment of a beta-galactosidase gene (beta GALA) to chromosome 3 in man. Cytogenetics and Cell Genetics. 22 (1–6) : 219–22. 2. Oshima A, Tsuji A, Nagao Y, Sakuraba H, Suzuki Y (Nov 1988) . Cloning, sequencing, and expression of cDNA for human beta-galactosidase. Biochemical and Biophysical Research Communications. 157 (1) : 238–44. 3. Caciotti A, Donati MA, Boneh A, d'Azzo A, Federico A, Parini R, Antuzzi D, Bardelli T, Nosi D, Kimonis V, Zammarchi E, Morrone A (Mar 2005) . Role of beta-galactosidase and elastin binding protein in lysosomal and nonlysosomal complexes of patients with GM1-gangliosidosis. Human Mutation. 25 (3) : 285–92.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

18080 MW

Observed Molecular Weight

65 kDa, 76 kDa, 85 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Galactosidase, beta 1

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Protein Name

Thy-1 membrane glycoprotein

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

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