AKR1C1 Recombinant Mouse Monoclonal Antibody [5-F10-R]
Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH. Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens. May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate. Displays affinity for bile acids.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
Swiss Prot
Q04828 Human
Cellular Locus
Cytoplasm.
Host
Mouse
Species Reactivity
Human
Immunogen
Recombinant protein within Human AKR1C1 aa 180-323 / 323.
Isotype
IgG1
Clone
5-F10-R
Conjugation
Non-conjugated
Type
Recombinant Mouse monoclonal Antibody
Applications
WB, IF-Cell
Positive Control
HepG2 cell lysate, U-87 MG cell lysate, HeLa cell lysate, A431 cell lysate, human liver tissue lysate, HeLa.
Concentration
1 mg/mL
Dilution
WB: 1:1,000 ;IF-Cell: 1:100
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 36.8 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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