AKR1C1 Recombinant Mouse Monoclonal Antibody [5-F10-R]
Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH. Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens. May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate. Displays affinity for bile acids.
Product Specifications
Product Name Alternative
Abbreviation
Swiss Prot
Q04828 Human
Cellular Locus
Cytoplasm.
Host
Mouse
Species Reactivity
Human
Immunogen
Recombinant protein within Human AKR1C1 aa 180-323 / 323.
Isotype
IgG1
Clone
5-F10-R
Conjugation
Non-conjugated
Type
Recombinant Mouse monoclonal Antibody
Applications
WB, IF-Cell
Positive Control
HepG2 cell lysate, U-87 MG cell lysate, HeLa cell lysate, A431 cell lysate, human liver tissue lysate, HeLa.
Concentration
1 mg/mL
Dilution
WB: 1:1,000 ;IF-Cell: 1:100
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 36.8 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
CAS Number
9000-83-3
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