Anti-ROC1/RBX1 Antibody Picoband® Fluoro488 Conjugated

RING-box protein 1, also known as ROC1, is a protein that in humans is encoded by the RBX1 gene. This gene is mapped to chromosome 22q13.2 based on an alignment of the RBX1 sequence with the genomic sequence. ROC1 is recruited by cullin-1 to form a quaternary SCF (HOS) -ROC1 holoenzyme (with SKP1 and the BTRCP homolog HOS) . SCF (HOS) -ROC1 binds IKK-beta-phosphorylated I-kappa-B-alpha and catalyzes its ubiquitination in the presence of ubiquitin, E1, and CDC34. Conclusively, ROC1 plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization.

E3 ubiquitin-protein ligase RBX1;6.3.2.-; Protein ZYP; RING finger protein 75; RING-box protein 1; Rbx1; Regulator of cullins 1; E3 ubiquitin-protein ligase RBX1, N-terminally processed; RBX1; RNF75, ROC1

Cell Biology, Epigenetics and Nuclear Signaling, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, SCF Complex E3 Ligase, Ubiquitin & Ubiquitin Like Modifiers, Ubiquitin E3 Enzymes

E3 ubiquitin ligase component of multiple cullin-RING- based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. .

1. Entrez Gene: RBX1 ring-box 1. 2. Kamura T, Koepp DM, Conrad MN, Skowyra D, Moreland RJ, Iliopoulos O, Lane WS, Kaelin WG Jr, Elledge SJ, Conaway RC, Harper JW, Conaway JW (May 1999) . Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase. Science 284 (5414) : 657–61. 3. Ohta T, Michel JJ, Schottelius AJ, Xiong Y (May 1999) . ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Mol Cell 3 (4) : 535–41.