Anti-GFP (N86/38) Recombinant Chicken Chimeric mAb FL594 Conjugate

Our chicken Anti-GFP recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N86/38. It detects GFP and is purified by affinity chromatography. It works in WB, IHC, ICC, IP, ELISA.

Product Specifications

Background

Green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is widely used as a marker of protein expression or targeting in cells or model organisms.

Short Description

Our chicken Anti-GFP FL594 conjugate recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N86/38. It detects n/a GFP and is purified by affinity chromatography. It works in IHC, ICC.

Synonyms

Green fluorescent protein

Gene Name

GFP

Host

Chicken

Immunogen

Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli

Target

GFP

Clonality

Recombinant

Clone

N86/38

Conjugation

FL594 Ex: 594 nm, Em: 615 nm

Applications

IHC, ICC

Field of Research

Quality Control: ICC/Anti-Tag Antibodies/

Assay Type

Primary Antibodies

Concentration

0.5 mg/mL

Format

Purified by affinity chromatography

Form

Liquid

pH

7.4

Molecular Weight

27 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N86/38 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N86/38 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

IHC Dilution

1:500-1:1000

Specificity

Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively) Does not cross-react with Red fluorescent protein (dsRed)

ICC Dilution

1:500-1:1000

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Uniprot Accession Number

P42212

Isotype

IgY

Immunogen Species

Jellyfish

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