JQ1-FITC

JQ1-FITC, a fluorescent BET bromodomain probe, exhibits high affinity binding to BRD4 (BD1) and BRD4 (BD2), with KD, app values of 6.5 nM and 5.8 nM respectively, as determined using GST-tagged BRD4 proteins in a CoraFluorTR-FRET assay; for BRD4 (BD1), the value is 58.7 nM in a fluorescence anisotropy assay. It has excitation and emission maxima (λ) of 495 nm and 525 nm.

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