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Monoclonal Anti-GFP Tag

Product Specifications

Background

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human.

Host

Mouse

Reactivity

All

Immunogen

Recombinant GFP protein with full length.

Applications

Western Blot (1:1000-1:10000) ; Immunoprecipitation, IP (1:100-1:1000) ; Immunofluorescence, IF (1:100-1:1000)

Form

Liquid

References & Citations

1. Prendergast F, Mann K (1978) . "Chemical and physical properties of aequorin and the green fluorescent protein isolated from Aequorea forskålea". Biochemistry 17 (17) : 3448–53. Tsien R (1998) . "The green fluorescent protein". Annu Rev Biochem 67: 509–44.

Storage Conditions

Store at -20°C for 1 year. Or aliquot and store at -80°C for long term. Avoid repeated freeze and thaw cycles.

Specificity

Recognize GFP and GFP fusion proteins.

Isotype

IgG1

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