Monoclonal Anti-GFP Tag
Product Specifications
Background
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human.
Host
Mouse
Reactivity
All
Immunogen
Recombinant GFP protein with full length.
Applications
Western Blot (1:1000-1:10000) ; Immunoprecipitation, IP (1:100-1:1000) ; Immunofluorescence, IF (1:100-1:1000)
Form
Liquid
References & Citations
1. Prendergast F, Mann K (1978) . "Chemical and physical properties of aequorin and the green fluorescent protein isolated from Aequorea forskålea". Biochemistry 17 (17) : 3448–53. Tsien R (1998) . "The green fluorescent protein". Annu Rev Biochem 67: 509–44.
Storage Conditions
Store at -20°C for 1 year. Or aliquot and store at -80°C for long term. Avoid repeated freeze and thaw cycles.
Specificity
Recognize GFP and GFP fusion proteins.
Isotype
IgG1
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