Recombinant Mouse IL-1a Protein

Recombinant mouse IL-1a is produced by the E.coli system, with the target gene encoding Ser115-Ser270 expressed.

Product Specifications

Background

Interleukin-1 alpha (IL-1 alpha) also known as hematopoietin 1 is a cytokine of the interleukin 1 family that in humans is encoded by the IL1A gene.In general, Interleukin 1 is responsible for the production of inflammation, as well as the promotion of fever and sepsis. IL-1α inhibitors are being developed to interrupt those processes and treat diseases.IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha.

Synonyms

IL1A, IL-1A, IL1, IL1-ALPHA, IL1F1, interleukin 1 alpha, IL-1 alpha, IL-1α

Source

E.coli

Applications

SDS-PAGE

Endotoxin

<1 EU/µg by LAL test

Purity

>95%by SDS-PAGE

Buffer

Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 6%Sucrose, 2%Mannitol, 50mM Arginine, 0.03% Tween80, pH 8.0.

Reconstitution

Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration<100μg/ml.)

References & Citations

Nicklin MJ, Weith A, Duff GW (Jan 1994) . "A physical map of the region encompassing the human interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist genes". Genomics. 19 (2) : 382–4. March CJ, Mosley B, Larsen A, Cerretti DP, Braedt G, Price V, et al. (August 1985) . "Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs". Nature. 315 (6021) : 641–7.