Recombinant human 14-3-3 zeta protein

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 zeta interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Recombinant human YWHAZ, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

YWHAZ, YWHAD, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta/delta, 14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

32 kDa (282aa) confirmed by MALDI-TOF

Additionnal Information

1433 zeta, YWZ0801-10 µg, YWZ0801-20 µg, YWZ0801-50 µg, YWZ0801-100 µg, YWZ0801-250 µg, YWZ0801-500 µg, YWZ0801-1 mg, YWZ0801-10, YWZ0801-20, YWZ0801-50, YWZ0801-100, YWZ0801-250, YWZ0801-500, YWZ0801-1

References & Citations

Gannon-Murakami L., et al. (2002) J Biol Chem. 277 (26) : 23116-23122; ; Li FQ., et al. (2008) J Cell Biol. 181 (7) :1141-54;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Signal Transduction

NCBI Accession Number

NP_663723

Uniprot Accession Number

P63104

Species

Human

AA Sequence

MDK NELVQKAKLA EQAERYDDMA ACMKSVTEQG AELSNEERNL LSVAYKNVVG ARRSSWRVVS SIEQKTEGAE KKQQMAREYR EKIETELRDI CNDVLSLLEK FLIPNASQAE SKVFYLKMKG DYYRYLAEVA AGDDKKGIVD QSQQAYQEAF EISKKEMQPT HPIRLGLALN FSVFYYEILN SPEKACSLAK TAFDEAIAEL DTLSEESYKD STLIMQLLRD NLTLWTSDTQ GDEAEAGEGG EN