Recombinant human SNAPIN protein

SNAPAP (SNAP associated protein) was enriched in neurons and exclusively located on synaptic vesicle membrane protein. SNAPAP is an important component of the neurotransmitter release process through its modulation of the sequential interactions between the SNAREs and synaptotagmin, which is a component of the SNARE complex that is required for synaptic vesicle docking and fusion. Recombinant human SNAPAP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

SNARE-associated protein Snapin, SNAP associated protein, Synaptosomal-associated protein 25-binding protein, SNAP25BP, SNAPAP, Biogenesis of lysosomal organelles complex-1 subunit 7, BLOC1S7, BORCS3, BLOC-1 related complex subunit 3, SNAP-25-binding protein

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

17 kDa (156aa) confirmed by MALDI-TOF

Additionnal Information

Snapin, SNAPAP, SNAP associated protein, SNAPIN, SNAP25BP, SNAPAP, SNAP associated protein, SNAP associated protein SNARE associated protein snapin, Synaptosomal associated protein 25 binding protein, SNA0901-10 µg, SNA0901-20 µg, SNA0901-50 µg, SNA0901-100 µg, SNA0901-250 µg, SNA0901-500 µg, SNA0901-1 mg, SNA0901-10, SNA0901-20, SNA0901-50, SNA0901-100, SNA0901-250, SNA0901-500, SNA0901-1

References & Citations

Ilardi JM., et al. (1999) . Nat Neurosci. 2 (2) :119-124; ; Chheda MG., et al. (2001) . Nat Cell Bioli. 3 (4) :331-8;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 2mM EDTA, 0.2 M NaCl2, 40% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_036569

Uniprot Accession Number

O95295

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MAGAGSAAVS GAGTPVAGPT GRDLFAEGLL EFLRPAVQQL DSHVHAVRES QVELREQIDN LATELCRINE DQKVALDLDP YVKKLLNARR RVVLVNNILQ NAQERLRRLN HSVAKETARR RAMLDSGIYP PGSPGK