Fibronectin 1 Antibody / FN1

Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Epitope of this MAb is located in the 2nd-3rd type-III repeats of fibronectin. Fibronectins are extracellular matrix glycoproteins that are essential for embryonic development. Fibronectins are also involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb reacts with human cellular fibronectin, but not plasma fibronectin.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P02751

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 467-595 was used as the immunogen for the Fibronectin 1 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

FN1/3569

Applications

ELISA, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Fibronectin 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Fibronectin 1 antibody is available for research use only.

Storage Conditions

Aliquot the Fibronectin 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Fibronectin 1 antibody should be determined by the researcher.

Location

Connective tissue matrix

Image Legend

IHC staining of FFPE human lung tissue with Fibronectin 1 antibody (clone FN1/3569) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.