Recombinant human CD158a/KIR2DL1 protein

An inhibitory Keller Cell Ig-like Receptor (KIR, previously called p58 KIR, p58. 1, cl-42, NKAT1, or KIR-K6), which recognizes class I MHC molecules (HLA-Cw2, -Cw4, -Cw5, and Cw6) . The protein coding region of the extracellular domain of KIR2DL1 (amino acids 1-202) was cloned into an E. coli expression vector. The extracellular domain of KIR2DL1 was overexpressed as insoluble protein aggregates (inclusion bodies) . The recombinant KIR2DL1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer.

Product Specifications

Product Name Alternative

Killer cell immunoglobulin like receptor two Ig domains and long cytoplasmic tail 1, Killer cell immunoglobulin-like receptor 2DL1, CD158 antigen-like family member A, Natural killer-associated transcript 1, NKAT-1, p58 natural killer cell receptor clones CL-42/47.11, p58 NK receptor CL-42/47.11, p58.1 MHC class-I-specific NK receptor, CD158a

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

22.2 kDa (202aa) confirmed by MALDI-TOF

Additionnal Information

KIR2DL1, CD158a, Killer cell immunoglobulin-like receptor 2DL1, KIR2DL1, CD158A, NKAT1, KIR2DL1 (p58 KIR, CD158), MHC class I NK cell receptor, Natural killer-associated transcript 1, NKAT-1, p58 natural killer cell receptor clones CL-42/47.11, p58 NK receptor, p58.1 MHC class-I-specific NK receptor, Killer cell immunoglobulin-like receptor 2DL1, Killer cell immunoglobulin-like receptor 2DL1 Killer cell immunoglobulin like receptor 2DL1, MHC class I NK cell receptor, Natural killer associated transcript 1, NKAT 1, p58 NK receptor, p58.1 MHC class I specific NK receptor, KIR3001-10 µg, KIR3001-20 µg, KIR3001-50 µg, KIR3001-100 µg, KIR3001-250 µg, KIR3001-500 µg, KIR3001-1 mg, KIR3001-10, KIR3001-20, KIR3001-50, KIR3001-100, KIR3001-250, KIR3001-500, KIR3001-1

References & Citations

Steffens, u. et al. (1998) Tissue Antigens 51, 398-413.; ; Wagtmann, N.R., et al. (1995) Immunity 2, 439-449.; ; Colonna, M. et al. (1995) Science 269, 405-408; ; ; ; Kim, J. et al. (1997) J. Immunol. 159, 3875-3882.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5)

Scientific Category

Immunology

NCBI Accession Number

NP_055033

Uniprot Accession Number

P43626

Species

Human

AA Sequence

MEGVHRKPSL LAHPGRLVKS EETVILQCWS DVMFEHFLLH REGMFNDTLR LIGEHHDGVS KANFSISRMT QDLAGTYRCY GSVTHSPYQV SAPSDPLDIV IIGLYEKPSL SAQLGPTVLA GENVTLSCSS RSSYDMYHLS REGEAHERRL PAGPKVNGTF QADFPLGPAT HGGTYRCFGS FHDSPYEWSK SSDPLLVSVT GN