Recombinant human IL-33 protein
Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine that may also regulate gene transcription in producer cells. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1. Recombinant human IL-33 (C-terminal fragment) was expressed in E.coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human IL-33 in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.3 ng/mL.
Product Name Alternative
Interleukin-33, Interleukin-1 family member 11, IL-1F11, Nuclear factor from high endothelial venules, NF-HEV, DVS27-related protein, DVS27, C9orf26
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.3 ng/mL.
Molecular Weight
18.1 kDa (160aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Schmitz., et al. (2005) immunity 23 (5) :479-90; ; Baekkevold, E.S., et al. (2003) Am. J.Pathol. 163 (1) :69-90;
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0)
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_254274
Uniprot Accession Number
O95760
Species
Human
AA Sequence
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