Recombinant human HTRA2/OMI (S306A) protein

HtrA2/Omi is a mammalian serine protease at high temperatures and has a chaperone activity at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Mature HtrA2 consists of a putative transmembrane domain; an inhibitor of apoptosis protein (IAP) -binding motif; a single C-terminal PDZ domain that mediates protein-protein interactions. Recently, HtrA2 has known to contribute both to caspase-dependent and caspase-independent cell death. Mature form of HtrA2/Omi (residues 134-458) was overexpressed in E. coli, and purified by conventional column chromatography techniques.

Product Specifications

Product Name Alternative

HtrA serine peptidase 2, High temperature requirement protein A2, HtrA2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI, PRSS25, OMI, PARK13

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

36 kDa (334aa) confirmed by MALDI-TOF

Additionnal Information

HtrA2, Omi (S306A), High temperature requirement protein A2, HTRA2, OMI, PRSS25, HtrA2/Omi, EC 3.4.21.108, High temperature requirement protein A2, HtrA2, Omi stress-regulated endoprotease, Serine proteinase OMI, Serine protease 25, Serine protease HTRA2, mitochondrial, High temperature requirement protein A2, HTR0501-10 µg, HTR0501-20 µg, HTR0501-50 µg, HTR0501-100 µg, HTR0501-250 µg, HTR0501-500 µg, HTR0501-1 mg, HTR0501-10, HTR0501-20, HTR0501-50, HTR0501-100, HTR0501-250, HTR0501-500, HTR0501-1

References & Citations

Savopoulos JW., et al., (2000) Protein Expr. Purif. 19, 227-34; ; Gray CW, et al., (2000) Eur. J. Biochem. 267, 5699-710 ; ; Martins LM, et al., (2002) J. Biol. Chem. 277, 439-44; ; Van Loo G, et al., (2002) Cell Death Differ. 9, 20-60;