Rabbit anti-NEFH Antibody
Product Specifications
Background
Rabbit polyclonal antibody to NEFH
Synonyms
KIAA0845; NFH; Neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; Neurofilament triplet H protein
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human NEFH
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of NEFH expression in mouse brain (A) whole cell lysates.
IHC description
Immunohistochemical analysis of NEFH staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
4744
Gene ID (Mouse)
380684
SwissProt (Human)
P12036
SwissProt (Mouse)
P19246
SwissProt (Rat)
P16884
CAS Number
9007-83-4
Available Sizes
Curated Selection
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