Recombinant human Frizzled-10 protein

Frizzled-10, also known as CD350, is a member of the G-protein coupled receptor Fz/Smo family. Frizzled-10 is expressed during embryogenesis in the primitive streak, dorsal neural tube, developing brain, limb bud, and airway epithelium. In the adult, it is expressed in placenta, gastric glands, and colon and renal tubule epithelial cells. It is a receptor for Wnt proteins and up-regulated in some cancers and transformed cell lines. Frizzled-10 associates with LRP5 to transduce Wnt 7a and Wnt 7b signals, resulting in the stabilization of cytoplasmic beta-catenin. Expression of this intronless gene is significantly up-regulated in two cases of primary colon cancer. It may also be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis or in differentiated tissues. Recombinant human Frizzled-10, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.