Recombinant human Ephrin-A5 protein
Ephrin-A5, as known as EFNA5, is a member of the ephrin ligand family which binds the members of ephrin receptor subfamily of tyrosine kinases. This protein is expressed with the highest levels in human adult brain, heart, spleen, and ovary and human fetal brain, lung, and kidney. It is also expressed by muscle precursor cells and interacts with ephrin-A4 to restrict their migration to the correct locations during forelimb morphogenesis. Recombinant human Ephrin-A5, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Mouse EphA3 (CAT# ATGP4148) is coated at 2 µg/mL (100 ul/well) can bind Human EFNA5. The ED50 range ≤ 60 ng/mL.
Product Name Alternative
Ephrin-A5, EFNA5, AF1, EFL5, EPLG7, GLC1M, LERK7, RAGS, AL-1, EPH-related receptor tyrosine kinase ligand 7
Expression System
Baculovirus
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Mouse EphA3 (CAT# ATGP4148) . The ED50 range ≤ 60 ng/mL.
Molecular Weight
48.1 kDa (422aa)
Additionnal Information
References & Citations
Son AI., et al. (2013) Mol. Vis. 19:254-266.; ; Wang TH., et al. (2012) FEBS J. 279:251-263.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_001953.1
Uniprot Accession Number
P52803
Species
Human
N Terminal Sequence
Gln 21
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