Recombinant mouse HPD protein
Hpd, also known as 4-hydroxyphenylpyruvate dioxygenase, is a Fe-containing enzyme that catalyzes the second reaction in the catabolism of tyrosine the conversion of 4-hydroxyphenylpyruvate to homogentisate. Existing as a homodimer, Hpd uses zinc as a cofactor to catalyze the third step in the conversion of L-phenylalanine to fumarate and acetoacetic acid. Defects in the gene encoding Hpd are the cause of tyrosinemia type 3 and hawkinsinuria, both of which are inborn errors of metabolism that are associated with a variety of symptoms, including mental retardation and seizures and hair and urine abnormalities. Recombinant mouse Hpd protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
4-hydroxyphenylpyruvate dioxygenase, 4HPPD, Fla, Flp, Hppd, Laf, 4-hydroxyphenylpyruvic acid oxidase, HPPDase, F Alloantigen, F protein
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Purity
> 90% by SDS-PAGE
Molecular Weight
47.4 kDa (416aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Wada GH., et al. (1975) J Biol Chem J. 250 (17) :6720-6.; ; Endo F., et al. (1997) J Biol Chem. 26; ;272 (39) :24426-32.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_032303
Uniprot Accession Number
P49429
Species
Mouse
AA Sequence
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