EGFR Antibody Cocktail
This mAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT
UniProt
P00533
Host
Mouse
Reactivity
Human
Immunogen
Human EGF Receptor purified from A431 cells (31G7) and recombinant human protein (GFR1195) were used as the immunogen for the EGFR antibody cocktail.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
31G7 + GFR1195
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the EGFR antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This EGFR antibody cocktail is available for research use only.
Storage Conditions
Store the EGFR antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cell surface
Image Legend
IHC: Formalin-fixed, paraffin-embedded human placenta stained with EGFR antibody (31G7 + GFR1195) .
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