Recombinant mouse Cyclophilin 40/PPID protein

Ppid, also known as Peptidyl-prolyl cis-trans isomerase D, is a member of peptidyl-propyl cis-trans isomerase (Pplase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. This protein can bind to the immunosuppressant cyclosporine A and was known that its overexpression the apoptosis in caner cells. Recombinant mouse Ppid protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin

Product Name Alternative

Peptidyl-prolyl cis-trans isomerase D, Peptidylprolyl isomerase D, Cyclophilin D, Cyclophilin 40, CYP-40, PPIase D, Rotamase D

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin

Molecular Weight

43.4 kDa (395aa) confirmed by MALDI-TOF

Additionnal Information

Cyclophilin40, Ppid, Peptidyl-prolyl cis-trans isomerase D, 4930564J03Rik, CYP-40, Ppid1, Ppif, ATGP3724-10 µg, ATGP3724-20 µg, ATGP3724-50 µg, ATGP3724-100 µg, ATGP3724-250 µg, ATGP3724-500 µg, ATGP3724-1 mg, ATGP3724-10, ATGP3724-20, ATGP3724-50, ATGP3724-100, ATGP3724-250, ATGP3724-500, ATGP3724-1

References & Citations

Yokoi H., et al. (1996) Genomics. 1;35 (3) :448-55. ; ; Tavecchio M., et al. (2013) J. Biol. Chem. 22;288 (8) :5553-61.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Cell Cycle

NCBI Accession Number

NP_080628

Uniprot Accession Number

Q9CR16

Species

Mouse

AA Sequence

MSHAS PAAKPSNSKN PRVFFDVDIG GERVGRIVLE LFADIVPKTA ENFRALCTGE KGTGSTTGKP LHFKGCPFHR IIKKFMIQGG DFSNQNGTGG ESIYGEKFED ENFHYKHDRE GLLSMANAGP NTNGSQFFIT TVPTPHLDGK HVVFGQVIKG LGVARTLENV EVNGEKPAKL CVIAECGELK EGDDWGIFPK DGSGDSHPDF PEDADIDLKD VDKILLISED LKNIGNTFFK SQNWEMAIKK YAKVLRYVDS SKAVIEKADR SRLQPIALSC VLNIGACKLK MSNWQGAIDS CLEALEMDPS NTKALYRKAQ GWQGLKEYDQ ALADLKKAQE IAPGDKAIQA ELLKVKQMIK AQKDKEKAVY AKMFA