Human Synaptotagmin-3 (SYT3) ELISA Kit

Synaptotagmin-3 (SYT3) is a Ca2+ sensor involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain. Ca2+ induces binding of the C2-domains to phospholipid membranes and to assembled SNARE-complexes; both actions contribute to triggering exocytosis. It plays a role in dendrite formation by melanocytes.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Synaptotagmin-3 (SYT3) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYT3. No significant cross-reactivity or interference between Human SYT3 and analogues was observed.

This Human Synaptotagmin-3 (SYT3) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT3 in samples. An antibody specific for SYT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.