Recombinant human P4HB protein

P4HB, prolyl 4 hydroxylase subunit beta, is a multifunctional and highly abundant enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This protein is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Recombinant human P4HB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity > 100 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.

Product Name Alternative

Proly 4-hydroxylase subunit beta, P4Hbeta, PDI, PDIA1, PHD, PO4DB, PO4HB; ERBA2L, Protein disulfide-isomerase, Cellular thyroid hormone-binding protein, p55

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity > 100 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.

Molecular Weight

57.5 kDa (521aa) confirmed by MALDI-TOF

Additionnal Information

P4HB, Proly 4-hydroxylase subunit beta, P4Hbeta, PDI, PDIA1, PHD, PO4DB, PO4HB; ERBA2L, ATGP3136-10 µg, ATGP3136-20 µg, ATGP3136-50 µg, ATGP3136-100 µg, ATGP3136-250 µg, ATGP3136-500 µg, ATGP3136-1 mg, ATGP3136-10, ATGP3136-20, ATGP3136-50, ATGP3136-100, ATGP3136-250, ATGP3136-500, ATGP3136-1

References & Citations

Pfander D., et al. (2006) Am J Pathol. 169 (2) :491-502.; ; Kivirikko KI., et al. (1991) J Hepatol. 13 Suppl 3:S2-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_000909

Uniprot Accession Number

P07237

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MDAPEEEDHV LVLRKSNFAE ALAAHKYLLV EFYAPWCGHC KALAPEYAKA AGKLKAEGSE IRLAKVDATE ESDLAQQYGV RGYPTIKFFR NGDTASPKEY TAGREADDIV NWLKKRTGPA ATTLPDGAAA ESLVESSEVA VIGFFKDVES DSAKQFLQAA EAIDDIPFGI TSNSDVFSKY QLDKDGVVLF KKFDEGRNNF EGEVTKENLL DFIKHNQLPL VIEFTEQTAP KIFGGEIKTH ILLFLPKSVS DYDGKLSNFK TAAESFKGKI LFIFIDSDHT DNQRILEFFG LKKEECPAVR LITLEEEMTK YKPESEELTA ERITEFCHRF LEGKIKPHLM SQELPEDWDK QPVKVLVGKN FEDVAFDEKK NVFVEFYAPW CGHCKQLAPI WDKLGETYKD HENIVIAKMD STANEVEAVK VHSFPTLKFF PASADRTVID YNGERTLDGF KKFLESGGQD GAGDDDDLED LEEAEEPDME EDDDQKAVKD EL