Recombinant human MRPL2 protein
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL2 is a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. Recombinant human MRPL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
39S ribosomal protein L2 mitochondrial, 39S ribosomal protein L2, mitochondrial, CGI-22, MRP-L14, RPML14
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
15.5 kDa (142aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
O'Brien TW, Fiesler SE, et al. (1999) . J Biol Chem. 274 (51) :36043-51.; ; Kenmochi N, Suzuki T, et al. (2001) . Genomics. 77 (1-2) :65-70.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Phosphate buffer (pH 8.0) containing 1mM EDTA, 50% glycerol, 2mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_057034
Uniprot Accession Number
Q5T653
Species
Human
AA Sequence
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