Recombinant human Sirtuin 5/SIRT5 protein
SIRT5 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants Recombinant human SIRT5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
NAD-dependent protein deacylase sirtuin-5 mitochondrial, Regulatory protein SIR2 homolog 5, SIR2-like protein 5, SIR2L5
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
32.5 kDa (300aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Zhou, Y., et al. (2012) J. Biol. Chem. 287 (34), 28307-28314; ; Fischer, F., et al. (2012) PLoS ONE 7 (9), E45098
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_036373.1
Uniprot Accession Number
Q9NXA8
Species
Human
AA Sequence
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