Recombinant human SEMG1 protein

SEMG1 is the predominant protein in semen. The secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. The prostate-specific antigen (PSA) protease processes this protein into smaller peptides, with each possibly having a separate function. The proteolysis process breaks down the gel matrix and allows the spermatozoa to move more freely. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human SEMG1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Semenogelin-1, Semenogelin 1, CT103, dJ172H20.2, SEMG, SGI

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

52 kDa (462aa)

Additionnal Information

SEMG1, Semenogelin-1, Semenogelin 1, CT103, dJ172H20.2, SEMG, SGI, ATGP2151-10 µg, ATGP2151-20 µg, ATGP2151-50 µg, ATGP2151-100 µg, ATGP2151-250 µg, ATGP2151-500 µg, ATGP2151-1 mg, ATGP2151-10, ATGP2151-20, ATGP2151-50, ATGP2151-100, ATGP2151-250, ATGP2151-500, ATGP2151-1

References & Citations

Brillard-Bourdet M, et al. (2002) . Eur J Biochem. 269 (1) :390-5

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.5M NaCl, 10% glycerol, 250 mM Imidazole, 0.1mM PMSF, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002998

Uniprot Accession Number

P04279

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSQKGGSKG RLPSEFSQFP HGQKGQHYSG QKGKQQTESK GSFSIQYTYH VDANDHDQSR KSQQYDLNAL HKTTKSQRHL GGSQQLLHNK QEGRDHDKSK GHFHRVVIHH KGGKAHRGTQ NPSQDQGNSP SGKGISSQYS NTEERLWVHG LSKEQTSVSG AQKGRKQGGS QSSYVLQTEE LVANKQQRET KNSHQNKGHY QNVVEVREEH SSKVQTSLCP AHQDKLQHGS KDIFSTQDEL LVYNKNQHQT KNLNQDQQHG RKANKISYQS SSTEERRLHY GENGVQKDVS QSSIYSQTEE KAQGKSQKQI TIPSQEQEHS QKANKISYQS SSTEERRLHY GENGVQKDVS QRSIYSQTEK LVAGKSQIQA PNPKQEPWHG ENAKGESGQS TNREQDLLSH EQKGRHQHGS HGGLDIVIIE QEDDSDRHLA QHLNNDRNPL FT