Recombinant human Amnionless protein

AMN, also known as amnionless, is a type I transmembrane protein. A complex of amnionless and cubilin forms the cubam receptor. This protein is necessary for efficient absorption of vitamin B12. It is thought to modulate bone morphogenetic protein (BMP) receptor function by serving as an accessory or coreceptor, and thus facilitates or hinders BMP binding. Recombinant human AMN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Protein amnionless, PRO1028

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

38.2 kDa (361aa) confirmed by MALDI-TOF

Additionnal Information

Amnionless, protein amnionless, PRO1028, ATGP1866-10 µg, ATGP1866-20 µg, ATGP1866-50 µg, ATGP1866-100 µg, ATGP1866-250 µg, ATGP1866-500 µg, ATGP1866-1 mg, ATGP1866-10, ATGP1866-20, ATGP1866-50, ATGP1866-100, ATGP1866-250, ATGP1866-500, ATGP1866-1

References & Citations

Fyfe J.C., et al. (2004) Blood 103:1573-1579; ; Tomihara-Newberger, C., et al. (1999) Dev. Biol. 204: 34-54.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_112205

Uniprot Accession Number

Q9BXJ7

Species

Human

AA Sequence

VSKLWVP NTDFDVAANW SQNRTPCAGG AVEFPADKMV SVLVQEGHAV SDMLLPLDGE LVLASGAGFG VSDVGSHLDC GAGEPAVFRD SDRFSWHDPH LWRSGDEAPG LFFVDAERVP CRHDDVFFPP SASFRVGLGP GASPVRVRSI SALGRTFTRD EDLAVFLASR AGRLRFHGPG ALSVGPEDCA DPSGCVCGNA EAQPWICAAL LQPLGGRCPQ AACHSALRPQ GQCCDLCGAV VLLTHGPAFD LERYRARILD TFLGLPQYHG LQVAVSKVPR SSRLREADTE IQVVLVENGP ETGGAGRLAR ALLADVAENG EALGVLEATM RESGAHVWGS S