Recombinant human SIAH1 protein
E3 ubiquitin-protein ligase SIAH1, also known as SIAH1, is a member of the seven in absentia homolog (SIAH) family. SIAH1 is a tumor suppressor protein that is expressed in intestinal epithelium and activated during apoptosis. SIAH1 contains an N-terminal RING-finger domain, which is required for proteolysis, and a cystein-rich C-terminal domain, which regulates oligomerization and SIAH binding to target proteins. SIAH1 is known to cause indirect degradation of beta-catenin through formation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi. Recombinant human SIAH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
E3 ubiquitin-protein ligase SIAH1, SIAH1A
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
24.1 kDa (216aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hu G., et al. (1999) Mol Cell Biol. 19: 724-732; ; Matsuzawas S., et al. (1998) EMBO J. 17: 2736-2747.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 40% glycerol,1mM DTT
Scientific Category
Ubiquitination
NCBI Accession Number
NP_003022
Uniprot Accession Number
Q8IUQ4
Species
Human
AA Sequence
Frequently Asked Questions
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