Anti-RABBIT IgG (H&L) (GUINEA PIG) Antibody
Product Specifications
CAS Number
9007-83-4
Reactivity
Rabbit
Immunogen
Rabbit IgG whole molecule
Target
Anti-Rabbit IgG (H&L) generated in guinea pig detects rabbit Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Secondary Antibody
Applications
CUT&RUN|Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1.21 mg/ml
Format
Liquid (sterile filtered)
Reconstitution
2°C to 8°C|-20°C
Fragment
IgG
Specificity
IgG (H&L) . Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Guinea Pig Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Goat, Human and Mouse Serum Proteins.
Host or Source
Guinea Pig
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