Recombinant human CMBL protein

Carboxymethylenebutenolidase homolog, also known as CMBL, is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL is the human homolog of Pseudomonas dienelactone hydrolase, a protein that participates in the bacterial halocatechol degradation pathway. CMBL is inhibited by p-chloromercuribenzoate (PCMB) and is encoded by a gene that maps to human chromosome 5p15. 2. Recombinant human CMBL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Carboxymethylenebutenolidase homolog

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

30.6 kDa (269aa) confirmed by MALDI-TOF

Additionnal Information

CMBL, Carboxymethylenebutenolidase homolog, ATGP1648-10 µg, ATGP1648-20 µg, ATGP1648-50 µg, ATGP1648-100 µg, ATGP1648-250 µg, ATGP1648-500 µg, ATGP1648-1 mg, ATGP1648-10, ATGP1648-20, ATGP1648-50, ATGP1648-100, ATGP1648-250, ATGP1648-500, ATGP1648-1

References & Citations

Ravandi F., et al. (2009) Cancer. 115: 5746-5751; ; Ishizuka T., et al. (2010) J Biol Chem. 285: 11892-11902.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM EDTA

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_620164.1

Uniprot Accession Number

Q96DG6

Species

Human

AA Sequence

MANEAY PCPCDIGHRL EYGGLGREVQ VEHIKAYVTK SPVDAGKAVI VIQDIFGWQL PNTRYIADMI SGNGYTTIVP DFFVGQEPWD PSGDWSIFPE WLKTRNAQKI DREISAILKY LKQQCHAQKI GIVGFCWGGT AVHHLMMKYS EFRAGVSVYG IVKDSEDIYN LKNPTLFIFA ENDVVIPLKD VSLLTQKLKE HCKVEYQIKT FSGQTHGFVH RKREDCSPAD KPYIDEARRN LIEWLNKYM