Recombinant human FUBP1 protein

FUBP1 is a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase. Recombinant human FUBP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Far upstream element (FuSE) binding protein 1, FBP, FuBP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by BCA assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

20.8 kDa (195aa) confirmed by MALDI-TOF

Additionnal Information

FUBP1, Far upstream element (FuSE) binding protein 1, FBP, FuBP, ATGP1587-10 µg, ATGP1587-20 µg, ATGP1587-50 µg, ATGP1587-100 µg, ATGP1587-250 µg, ATGP1587-500 µg, ATGP1587-1 mg, ATGP1587-10, ATGP1587-20, ATGP1587-50, ATGP1587-100, ATGP1587-250, ATGP1587-500, ATGP1587-1

References & Citations

Zheng, Y., et al. (2011) Int. J. Biochem. Cell Biol. 43 (11), 1641-1648; ; Neubauer, A., et al. (2011) Exp. Hematol. 39 (10), 1030-1042.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_003893.2

Uniprot Accession Number

Q96AE4

Species

Human

AA Sequence

DVPIP RFAVGIVIGR NGEMIKKIQN DAGVRIQFKP DDGTTPERIA QITGPPDRCQ HAAEIITDLL RSVQAGNPGG PGPGGRGRGR GQGNWNMGPP GGLQEFNFIV PTGKTGLIIG KGGETIKSIS QQSGARIELQ RNPPPNADPN MKLFTIRGTP QQIDYARQLI EEKIG