Recombinant mouse TNF-alpha protein
TNFa, also known as tumor necrosis factor, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia. Recombinant mouse TNFa protein was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF
Expression System
E.coli
Antigen Species
Mouse
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
17.4 kDa (157aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Shakhov A.N., et al. (1987) Bioorg. Khim. 13:701-705; ; Pennica D., et al. (1985) Proc. Natl. Acad. Sci. u.S.A. 82:6060-6064
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_038721
Uniprot Accession Number
P06804
Species
Mouse
AA Sequence
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
