Recombinant human MANF protein

Mesencephalic astrocyte-derived neurotrophic factor, also known as MANF, is a 20 kDa member of the ARMET family of proteins. MANF was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress. Recombinant human MANF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Mesencephalic astrocyte-derived neurotrophic factor, ARMET, ARP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

20.8 kDa (183aa) confirmed by MALDI-TOF

Additionnal Information

MANF, Mesencephalic astrocyte-derived neurotrophic factor, ARMET, ARP, ATGP1465-10 µg, ATGP1465-20 µg, ATGP1465-50 µg, ATGP1465-100 µg, ATGP1465-250 µg, ATGP1465-500 µg, ATGP1465-1 mg, ATGP1465-10, ATGP1465-20, ATGP1465-50, ATGP1465-100, ATGP1465-250, ATGP1465-500, ATGP1465-1

References & Citations

Shridhar V., et al. (1996) Cancer Res. 56:5576.; ; Petrova P S., et al. (2003) J Mol Neurosci. 20:173.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Neuroscience

NCBI Accession Number

NP_006001

Uniprot Accession Number

P55145

Species

Human

AA Sequence

LRPGD CEVCISYLGR FYQDLKDRDV TFSPATIENE LIKFCREARG KENRLCYYIG ATDDAATKII NEVSKPLAHH IPVEKICEKL KKKDSQICEL KYDKQIDLST VDLKKLRVKE LKKILDDWGE TCKGCAEKSD YIRKINELMP KYAPKAASAR TDL